THE MINOR LAB Home of the Electrosome

Publications

2023

EMC chaperone-CaV structure reveals an ion channel assembly intermediate. Chen Z, Mondal A, Aberemane-Ali, F, Jang S, Niranjan S, Montaño JL, Zaro BW, Minor, D.L., Jr. Nature 619 410-419 (2023)
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Structural basis for CaVɑ 2δ:gabapentin binding. Chen Z, Mondal A, and Minor, D.L., Jr. Nature Struct. & Mol. Biol. 30 735-739 (2023)
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2022

Selective posttranslational inhibition of CaVβ1-associated voltage-dependent calcium channels with a functionalized nanobody. Morgenstern, T., Nirwan, N., Hernandez-Ochoa, E., Bibollet, H., Choudhury, P., Laloudakis, J., Ben-Johny, M., Bannister, R., Schneider, M., Minor, D.L., Jr., and Colecraft, H.M. Nature Communications 13:7556 (2022)
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Definition of a saxitoxin (STX) binding code enables discovery and characterization of the Anuran saxiphilin family. Chen, Z., Zakrzewska, S., Hajare, H., Alvarez-Bullya, A., Abderemane-Ali, F., Bogan, M., Ramirez, D., O’Connell, L.A., Du Bois, J., and Minor, D.L., Jr. Proceedings of the National Academy of Sciences, USA 119:e2210114119 (2022)
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Quaternary structure independent folding of voltage-gated ion channel pore domain subunits. Arrigoni, C., Lolicato, M., Shaya, D., Rohaim, A., Findeisen, F., Fong, L.-K., Colleran, C.M., Dominik, P., Kim, S.S., Schuermann, J., DeGrado, W.F., Grabe, M., Kossiakoff, A.A., and Minor, D.L., Jr. Nature Structural and Molecular Biology 29: 537-548 (2022)
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2021

Structural insights into the mechanisms and pharmacology of K2P potassium channels.  Natale, A.M., Deal, P.E., and Minor, D.L. Jr. Journal of Molecular Biology 433:166995 (2021)
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Production of K2P2.1 (TREK-1) for structural studies.  Lee, H., Lolicato, M., Arrigoni, C, and Minor, D.L., Jr. Methods in Enzymology 653 151-188 (2021)
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Differential effects of modified batrachotoxins on voltage-gated sodium channel fast and slow inactivation. MacKenzie, T.M.G., Abderemane-Ali, F., Garrison, C.E., Minor, D.L., Jr., and Du Bois, J. Cell Chemical Biology  Dec 24;S2451-9456(21)00517-1 (2021)
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Evidence that toxin resistance in poison birds and frogs is not rooted in sodium channel mutations and relies on ‘toxin sponge’ proteins. Abderemane-Ali, F., Rossen, N.D., Kobiela, M.E., Craig, R.A.II, Garrison, C.E., Chen, Z., O'Connell, L.A., Du Bois, J., Dumbacher, J.P., and Minor, D.L., Jr. J. Gen. Physiol    153:e202112872 (2021)
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2020

K2P channel C-type gating involves asymmetric selectivity filter order-disorder transitions. Lolicato, M., Natale, A.M., Abderemane-Ali, F., Crottes, D., Capponi, S., Duman, R. Wagner, A., Rosenberg, J.M., Grabe, M., Minor, D.L. Jr., Science Advances 6 eabc9174 (2020)
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Polynuclear Ruthenium Amines Inhibit K2P Channels via a "Finger in the Dam" Mechanism. Pope, L., Lolicato, M., Minor, D.L. Jr., Cell Chemical Biology   27, 511-524 (2020)
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2019

SARAF luminal domain structure reveals a novel domain-swapped β-sandwich fold important for SOCE modulation. Kimberlin, C.R.,Meshcheriakova, A., Palty, R., Karbat, I., Reuveny, E., and Minor, D.L. Jr. Journal of Molecular Biology 431 2869-2883 (2019)
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A selectivity filter gate controls voltage gated calcium channel (CaV) calcium-dependent inactivation. Abderemane-Ali, F., Findeisen, F., Rossen, N.D., and Minor, D.L. Jr., Neuron 101 1134-1149 (2019)
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Structure of the saxiphilin:saxitoxin (STX)complex reveals a convergent molecular recognition strategy for paralytic toxins. Yen, T.-J., Lolicato, M., Thomas-Tran, R., Du Bois, J., and Minor, D.L. Jr., Science Advances 5 eaax2650 (2019)
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2018

Protein and chemical determinants of BL-1249 action and selectivity for K2P channels. Pope. L., Arrigoni, C., Lou, H., Bryant, C. Gallardo-Gadoy, A., Renslo, A.R., and Minor, D.L. Jr., ACS Chemical Neuroscience 9 3153-3165, (2018)
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Structural basis for activity and specificity of an anticoagulant anti-FXIa monoclonal antibody and a reversal agent. Ely, L., Lolicato, M. David, T., Lowe, K., Kim, Y.C., Samuel, D., Bessette, P., Garcia, J.L., Mikita, T., Minor, D.L. Jr., Coughlin, S.R., Structure 26187-198 (2018)
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A Calmodulin C-Lobe Ca2+-Dependent Switch Governs Kv7 Channel Function. Chang, A., Abderemane-Ali, F., Hura, G.L., Rossen, N.D., Gate, R.E., Minor, D.L., Jr., Neuron 97 836-852 (2018)
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Global versus local mechanisms of temperature sensing in ion channels. Arrigoni, C. and Minor, D.L. Jr., Pflügers Archiv: European Journal of Physiology 470 733-744 (2018)
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2017

Channel surfing uncovers a dual-use transporter. Minor, D.L. Jr. The EMBO Journal 36 3272-3273 (2017)
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Cryo-EM structures of the TMEM16A calcium-activated chloride channel. Dang S., Feng S., Tien J., Peters C.J., Bulkley D., Lolicato M., Zhao J., Zuberbühler K., Ye W., Qi L., Chen T., Craik C.S., Nung Jan Y., Minor D.L. Jr, Cheng Y., Yeh Jan L., Nature 552 426-429 (2017)
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K2P2.1(TREK-1):activator complexes reveal a cryptic selectivity filter binding site. Lolicato, M., Arrigoni, C., Mori, T., Sekioka, Y., Bryant, C., Clark, K.A., Minor, D.L., Jr., Nature 547 364-368 (2017)
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Stapled voltage-gated calcium channel (CaV) α-Interaction Domain (AID) peptides act as selective protein-protein interaction inhibitors of CaV Function. Findeisen, F., Campiglio, M., Jo, H., Abderemane-Ali, F., Rumpf, C.H., Pope, L., Rossen, N.D., Flucher, B.E., DeGrado, W.F., and Minor D.L., Jr., ACS Chemical Neuroscience 8 1313-1326 (2017)
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Two P domain potassium channels, introduction. Plant, L.D., Bayliss, D.A., Minor, D.L. Jr., Czirják, G., Enyedi, P., Lesage, F., Sepúlveda, F., Goldstein, S.A.N.  IUPHAR/BPS Guide to Pharmacology (2017) 
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2016

Unfolding of a temperature-sensitive domain controls voltage-gated channel activation. Arrigoni, C., Rohaim, A., Shaya, D., Findeisen, F., Stein, R.A. Nurva, S.R., Mishra, S., Mchaourab, H.S., and Minor, D.L., Jr., Cell 164 922-936 (2016)
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Let it go and open up: An ensemble of ion channel active states. Minor, D.L., Jr., Cell 164 597-598 (2016)
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2015

Insights into the molecular foundations of electrical excitation. Gaudet, R., Roux, B, and Minor, and D.L., Jr., Journal of Molecular Biology 427 1-2 (2015)
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Bacterial voltage-gated sodium channels (BacNaVs) from the soil, sea, and salt lakes enlighten molecular mechanisms of electrical signaling and pharmacology in the brain and heart. Payandeh, J. and Minor, D.L., Jr., Journal of Molecular Biology 427 3-30 (2015)
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2014

Transmembrane helix straightening and buckling underlies activation of mechanosensitive and thermosensitive K2P channels. Lolicato, M., Riegelhaupt, P.M., Arrigoni, C., Clark, K.A., Minor, D.L., Jr., Neuron 84 1198-1212 (2014)
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Tethered protein display identifies a novel Kir3.2 (GIRK2) regulator from protein scaffold libraries. Bagriantsev, S.N, Chatelain, F.C., Clark, K.A., Alagem, N., Reuveny, E., and Minor, D.L., Jr., ACS Chemical Neuroscience 5 812-822 (2014)
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Structure of a prokaryotic sodium channel pore reveals essential gating elements and an outer ion binding site common to eukaryotic channels. Shaya, D., Findeisen, F., Abderemane-Ali, F., Arrigoni, C., Wong, S., Reddy Nurva, S., Loussouarn, G., and Minor, D.L., Jr. Journal of Molecular Biology 426 476-483 (2014)
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2013

Conduits of life's spark: A perspective on ion channel research since the birth of Neuron. Isacoff, E.Y., Jan, L.Y., and Minor, D.L., Jr., Neuron 80 658-674 (2013)
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Apo states of calmodulin and CaBP1 control CaV1 voltage-gated calcium channel function through direct competition for the IQ domain. Findeisen, F., Rumpf, C.H., and Minor, D.L., Jr., Journal of Molecular Biology 425 3217-3234 (2013)
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A high-throughput functional screen identifies small molecule regulators or temperature- and mechano-sensitive K2P channels. Bagriantsev, S. N., Ang, K.H., Gallardo-Godoy, A, Clark, K.A., Arkin, M.R., Renslo, A.R, and Minor, D.L., Jr., ACS Chemical Biology 8 1841-1851 (2013)
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Identification of a dimerization domain in the TMEM16A calcium-activated chloride channel (CaCC). Tien, J., Lee, H.Y., Minor, D.L., Jr., Jan, Y.N, and Jan, L.Y., Proc. Natl. Acad. Sci. USA 110 6352-6357 (2013)
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Patch-clamp characterization of the MscS-like mechanosensitive channel from Silicibacter pomeroyi. Petrov, E., Palanivelu, D., Nomura, T., Constantine, M., Rohde, P.R., Cox, C.D., Minor, D.L., Jr., and Martinac, B., Biophysical Journal 104 1426-1434 (2013)
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Using yeast to study potassium channel function and interactions with small molecules. Bagriantsev, S. and Minor, D.L., Jr., Methods in Molecular Biology 995 31-42 (2013)
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Structure of a Ca2+/CaM:Kv7.4 (KCNQ4) B-helix complex provides insight into M-current modulation. Xu, Q., Chang, A., Tolia, A., and Minor, D.L., Jr., Journal of Molecular Biology 425 378-394 (2013)
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2012

Metabolic and thermal stimuli control K2P2.1 (TREK-1) through modular sensory and gating domains. Bagriantsev, S., Clark, K.A., and Minor, D.L., Jr., The EMBO Journal 31 3297-3308 (2012)
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A glutamate switch controls voltage-sensitive phosphatase function. Liu L., Kohout, S.C., Xu, Q., Müller, S., Kimberlin, C.R., Isacoff, E.Y. Minor, D.L., Jr., Nature Structural and Molecular Biology 19 633-641 (2012)
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Chemical informatics and target identification in a zebrafish phenotypic screen. Laggner, C., Kokel, D., Setola, V., Tolia, A., Lin, H., Irwin, J.J., Keiser, M.J., Minor, D.L., Jr., Roth, B.L., Peterson, R.T., Shoichet, B.K., Nature Chemical Biology 8 144-146 (2012)
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2011

Multiple modalities converge on a common gate to control K2P channel function. Sviatoslav N Bagriantsev, Remi Peyronnet, Kimberly A Clark, Eric Honore, Daniel L Minor Jr., The EMBO Journal 30, 3594–3606 (2011)
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Voltage-gated sodium channel (NaV) protein dissection creates a set of functional pore-only proteins. David Shaya, Mohamed Kreir, Rebecca A. Robbins, Stephanie Wong, Justus Hammon, Andrea Brüggemann, and Daniel L. Minor, Jr., PNAS Proc Natl Acad Sci USA Jul 26;108(30):12313-8. (2011)
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Calmodulin overexpression does not alter CaV1.2 function or oligomerization state. Felix Findeisen, Alexandra Tolia, Ryan Arant, Eun Young Kim, Ehud Y. Isacoff and Daniel L. Minor, Jr., Channels 5:4, 320-324; (2011)
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2010

Progress in the structural understanding of voltage-gated calcium channel (CaV) function and modulation. Findeisen, Felix, Minor, Jr., D.L., Channels 4, 28-43 (2010)
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Structural Basis for the Differential Effects of CaBP1 and Calmodulin on CaV1.2 Calcium-Dependent Inactivation. Findeisen, F., Minor, Jr., D.L., Structure 18, 1617–1631 (2010)
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Multiple C-terminal tail Ca2+/CaMs regulate CaV1.2 function but do not mediate channel dimerization. Ki., E., Rumpf, C., Van Petegem, F., Arant, R.J., Findeisen, F., Cooley, E., Isacoff, E., Minor, Jr., D.L., The EMBO Journal 29, 3924-3938 (2010)
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Small molecule-ion channel match making: A natural fit for new ASIC ligands. Bagriantsev, S. and Minor, D.L., Jr., Neuron 68 1-3 (2010)

Calmodulin interactions with CaV1 and CaV2 voltage-gated calcium channel IQ domains. Kim, E., Findeisen, F., Minor, Jr., D.L. Handbook of Metalloproteins (2010)
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Electrochemical coupling in the voltage-dependent phosphatase Ci-VSP. Kohout, S., Bell, S.C., Liu, L., Xu, Q., Minor, Jr., D.L., Isacoff, E.Y, Nature Chemical Biology 6, 369-375 (2010)
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2009

An Overview of Ion Channel Structure. Published in the Handbook of Cell Signaling, 2nd Edition, Chapter author: Daniel L. Minor, Pages 201-208, Copyright Elsevier (2009)
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Selection of Inhibitor-Resistant Viral Potassium Channels Identifies a Selectivity Filter Site that Affects Barium and Amantadine Block. Chatelain, F.C., Gazzarrini, S., Fujiwara, Y., Arrigoni, C., Domigan, C., Ferrara, G., Pantoja, C., Thiel, G., Moroni, A., Minor, Jr., D.L., PLoS ONE Vol 4 Issue 10 e7496 (2009)
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Searching for interesting channels: pairing selection and molecular evolution methods to study ion channel structure and function. Minor, Jr., D.L., Molecular BioSystems, 5, 802–810 (2009)
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Crystal structure of a trimeric form of the KV7.1 (KCNQ1) A-domain tail coiled-coil reveals structural plasticity and context dependent changes in a putative coiled-coil trimerization motif. Xu, Q., Minor, Jr., D.L., Protein Science 18:2100—2114 (2009)
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Disruption of the IS6-AID Linker Affects Voltage-gated Calcium Channel Inactivation and Facilitation. Findeisen, F., Minor, Jr., D.L.,The Journal of General Physiology 133 No. 3, 327-343 (2009)
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A green fluorescent protein screen for identification of well-expressed membrane proteins from a cohort of extremophilic organisms. Hammon, J., Palanivelu, D.V., Chen, J., Patel, C., Minor, Jr., D.L. Protein Science 18, 121-133 (2009)
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2008

Transmembrane domain length of viral K channels is a signal for mitochondria targeting. Balss, J., Papatheodorou, P., Mehmel, M., Baumeister, D., Hertel, B., Delaroque, N., Chatelain, F.C., Minor, Jr., D.L., Van Etten, J.L., Rassow, J., Moroni, A., Thiel, G., PNAS 34, 12313-12318 (2008)
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X-ray Crystal Structure of a TRPM Assembly Domain Reveals an Antiparallel Four-stranded Coiled-coil. Fujiwara, Y., Minor, Jr., D.L., J. Mol. Biol. 383, 854-870 (2008).
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Structures of CaV2 Ca2+/CaM-IQ Domain Complexes Reveal Binding Modes that Underlie Calcium-Dependent Inactivation and Facilitation. Kim, E.Y., Rumpf, C.H., Fujiwara, Y., Cooley, E.S., Van Petegem, F., and Minor, Jr., D.L., Structure 16, 1455-1467 (2008).
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Alanine-Scanning Mutagenesis Defines a Conserved Energetic Hotspot in the CaVα1 AID-CaVβ Interaction Site that Is Critical for Channel Modulation. Petegem, F.V., Duderstadt, K.E., Clark, K.A., Wang, M., Minor, Jr., D.L., Structure 16, 280-294 (2008)
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2007

Puzzle plugged by pore plasticity. Minor, Jr., D.L., Molecular Cell 26 459-460 (2007)
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The neurobiologist's guide to structural biology: A Primer on why macromolecular structure matters and how to evaluate macromolecular structural data. Minor, Jr., D.L., Neuron 54 511-533 (2007)
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Structural insight into KCNQ (Kv7) channel assembly and channelopathy. Howard, R.J., Clark, K.A., Holton, J.M., and Minor, Jr., D.L., Neuron 53 663-675 (2007)
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2006

A multimeric membrane protein reveals 14-3-3 isoform specificity in forward transport in yeast. Michelsen, K., Mrowiec, T., Duderstadt, K.E., Frey, S., Minor, Jr., D.L., Mayer, M.P., Schwappach, B., Traffic 7 903-916 (2006)
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Coiled-coils direct assembly of a cold-activated TRP channel. Tsuruda, P., Julius, D., and Minor, Jr., D.L., Neuron 51 201-212 (2006)
» Abstract at PubMed
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Three-dimensional structure of the KChIP/Kv4.3 T1 domain complex reveals a cross-shaped octamer. Pioletti, M., Findeisen, F., Hura, G.L., and Minor, Jr., D.L., Nature Structural & Molecular Biology (2006)
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A sensitive channel family replete with sense and motion. Minor, Jr., D.L., Nature Structural & Molecular Biology 13 388-390 (2006)
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Wanting contact: how to pick up a channel. Minor, Jr., D.L., Nature Chemical Biology 2 298-299 (2006)
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The structural biology of voltage-gated calcium channel function and regulation. Van Petegem, F. and Minor, Jr., D.L., Biochemical Society Transactions Vol 34, part 5 (2006)
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2005

Insights into voltage-gated calcium channel regulation from the structure of the CaV1.2 IQ domain-Ca2+/calmodulin complex. Van Petegem, F., Chatelain, F.C., and Minor, Jr., D.L., Nature Structural and Molecular Biology 12 1108-1115 (2005)
» Abstract at PubMed
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The pore helix dipole has a minor role in inward rectifier channel function. Chatelain, F.C., Alagem, N., Xu, Q., Pancaroglu, R., Reuveny, E., and Minor, Jr., D.L., Neuron 47, 833-843 (2005)
» Abstract at PubMed
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Bend to open? Minor, Jr., D.L., Structure 13 1094-1095 (2005)
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2000-2004

Overview: Function and three-dimensional structures of ion channels. Minor, Jr., D.L., Handbook of Cellular Signaling, Eds. R. Bradshaw and E. Dennis, Academic Press, San Diego (2004)
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Structure of a complex between a voltage gated calcium channel beta-subunit and an alpha-subunit domain. Van Petegem, F., Clark, K.A., Chatelain, F.C., and Minor, Jr., D.L., Nature 429 671-675 (2004)
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The structure of APPBP-1UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1. Walden, H., Podgorski, M.S., Huang, D.T., Miller, D.W., Howard, R.J., Minor, Jr., D.L., Holton, J.M., and Schulman, B.A., Molecular Cell 12 1427-1437 (2003)
» Abstract at PubMed
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Consensus-derived structural determinants of the ankyrin repeat motif. Mosavi, L. K., Minor, Jr., D.L., and Peng, Z.-y., Proceedings of the National Academy of Sciences USA 99 16029-16034 (2002)
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Potassium Channels: life in the post-structural world. Minor, Jr., D.L., Current Opinion in Structural Biology 11 408-414 (2001)
» Abstract at PubMed
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Controlling potassium channel activities: Interplay between the membrane and intracellular factors. Yi, B.A, Minor, Jr., D.L., Lin, Y.F., Jan, Y.N. and Jan, L.Y., Proceedings of the National Academy of Sciences USA 98 11016-11023 (2001)
» Abstract at PubMed
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The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel. Minor, Jr., D.L., Lin, Y.F, Mobley, B.C., Avelar, A., Jan, Y.N., Jan, L.Y. and Berger, J.M., Cell 102 657-670 (2000)
» Abstract at PubMed
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1994-1999

Transmembrane structure of an inwardly rectifying potassium channel. Minor, Jr., D.L., Masseling, S.J., Jan, Y.N. and Jan, L.Y., Cell 96 879-891 (1999)
» Abstract at PubMed
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Identification of (D)-peptide ligands through Mirror-Image phage display. Schumacher, T.N.M., Mayr, L.M., Minor, Jr., D.L., Milhollen, M.A., Burgess, M.W. and Kim, P.S., Science 271 1854-1857 (1996)
» Abstract at PubMed
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Context-dependent secondary structure formation of a designed protein sequence. Minor, Jr., D.L. and Kim P.S., Nature 380 730-734 (1996)
» Abstract at PubMed
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Measurement of the beta-sheet forming propensities of amino acids. Minor, Jr., D.L., and Kim P. S., Nature 367 660-663 (1994)
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Context is a major determinant of beta-sheet propensity. Minor, Jr., D.L. and Kim P.S., Nature 371 264-267 (1994)
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