Structural basis for activity and specificity of an anticoagulant anti-FXIa monoclonal antibody and a reversal agent. Ely, L., Lolicato, M. David, T., Lowe, K., Kim, Y.C., Samuel, D., Bessette, P., Garcia, J.L., Mikita, T., Minor, D.L. Jr., Coughlin, S.R., Structure 26187-198 (2018)
A Calmodulin C-Lobe Ca2+-Dependent Switch Governs Kv7 Channel Function. Chang, A., Abderemane-Ali, F., Hura, G.L., Rossen, N.D., Gate, R.E., Minor, D.L., Jr., Neuron 97 836-852 (2018) View Video Abstract
Global versus local mechanisms of temperature sensing in ion channels. Arrigoni, C., Minor, D.L., Jr., Pflügers Archiv-European Journal of Physiology 1-12 (2018) ONLINE
Cryo-EM structures of the TMEM16A calcium-activated chloride channel. Dang S., Feng S., Tien J., Peters C.J., Bulkley D., Lolicato M., Zhao J., Zuberbühler K., Ye W., Qi L., Chen T., Craik C.S., Nung Jan Y., Minor D.L. Jr, Cheng Y., Yeh Jan L., Nature 552 426-429 (2017)
K2P2.1(TREK-1):activator complexes reveal a cryptic selectivity filter binding site. Lolicato, M., Arrigoni, C., Mori, T., Sekioka, Y., Bryant, C., Clark, K.A., Minor, D.L., Jr., Nature 547 364-368 (2017)
Stapled voltage-gated calcium channel (CaV) α-Interaction Domain (AID) peptides act as selective protein-protein interaction inhibitors of CaV Function. Findeisen, F., Campiglio, M., Jo, H., Abderemane-Ali, F., Rumpf, C.H., Pope, L., Rossen, N.D., Flucher, B.E., DeGrado, W.F., and Minor D.L., Jr., ACS Chemical Neuroscience 8 1313-1326 (2017)
Unfolding of a temperature-sensitive domain controls voltage-gated channel activation. Arrigoni, C., Rohaim, A., Shaya, D., Findeisen, F., Stein, R.A. Nurva, S.R., Mishra, S., Mchaourab, H.S., and Minor, D.L., Jr., Cell 164 922-936 (2016)
MINOR LAB RESEARCH
Our lab employs a range of biochemical, biophysical, structural, and chemical biology approaches to examine the molecular structures and functions of various classes of ion channels including members of the voltage-gated potassium, voltage-gated calcium, voltage-gated sodium, and K2P channel families. Our research relies heavily on X-ray crystallography, cryo-electron microscopy, isothermal titration calorimetry, circular dichroism, and other biophysical methods. Because ion channel structure is intimately tied to function, an equally crucial part of our efforts implements structure-based tests of ion channel function using electrophysiological recordings in live cells.
Because most channels suffer from poor pharmacological profiles that limit the ability to connect ion channel genes with their physiological functions, our lab also has a strong effort to develop novel ion channel modulators. The development of new selective inhibitors and activators of channel function should provide new tools for ion channel research and may lead to the development of novel, ion channel directed pharmaceuticals.
MINOR LAB LIFE
The Minor lab is located in the Smith Cardiovascular Research Building at the UCSF Mission Bay Campus in San Francisco. If you are interested in joining us, please see here.