The Black Lab

Protocols

• Cre genotyping
• lacZ genotyping
• Mef2c genotyping
• X-gal staining of embryos
• Transgene preparation
• Mouse CRISPR
• anti-ß-gal IHC
• embryo explant culture
• EMSA

Plasmid Maps
Select a plasmid map from the following list:

• HSP68-lacZ
• pAUG-ß-gal
• Mef2c-AHF-Cre
• Mef2c-AHF-lacZ

For additional plasmid requests, please click here to E-mail Brian Black.

Reagent Requests

For reagent or mouse requests, please click here to E-mail Brian Black.

If you have already made arrangements with Brian for a mouse shipment, please click here to E-mail Kachim Okpo to coordinate shipping. In the E-mail, please include the phone, fax, and E-mail for the PI, lab contact, veterinarian, and shipping coordinator.

Please note that Mef2c-73k-Cre (skeletal muscle Cre) and Mef2c-AHF-Cre (anterior heart field from the
male and universal deleter from the female) are available from the MMRRC through the following links:

• Mef2c-73k-Cre
• Mef2c-AHF-Cre