The Minor Lab merges structural, biochemical, genetic, and electrophysiological methods to dissect the mechanisms of action of ion channels, the complex protein machines that generate electrical signals in the heart, brain, and sensory systems.
Our research spans a range of eukaryotic membrane proteins important for brain and heart function including voltage-gated calcium channels and K2P channels, as well as bacterial ion channels such as BacNaVs (bacterial voltage-gated sodium channels). One of the BacNaVs we study, NaVAe, comes from an organism living in Mono Lake, California (top image on this page). The insights into human ion channel function derived from such studies underlines the deep importance of evolutionary connections among all forms of life that underpin modern biological science.
Research at UC San Francisco Connects Mono Lake, Evolution, and Cardiovascular Health by Julia Frankenback Dec. 8, 2013
UCSF Research Connects Mono Lake, Evolution, and Cardiovascular Health by Clarice Estrada Dec. 11, 2013
Daniel L. Minor, Jr., Ph.D.Professor
University of California San Francisco,
Cardiovascular Research Institute
Department of Biochemistry and Biophysics and Cellular and Molecular Pharmacology
Faculty Scientist, Molecular Biophysics & Integrated Imaging Division, Lawrence Berkeley National Laboratory, Berkeley
The Minor lab is located in the Smith Cardiovascular Research Building at the UCSF Mission Bay Campus in San Francisco. If you are interested in joining us, please see here.
SARAF luminal domain structure reveals a novel domain-swapped β-sandwich fold important for SOCE modulation. Kimberlin, C.R.,Meshcheriakova, A., Palty, R., Karbat, I., Reuveny, E., and Minor, D.L. Jr. Journal of Molecular Biology 431 2869-2883 (2019) PMID:31082439 PMCID: PMC6599547
A selectivity filter gate controls voltage gated calcium channel (CaV) calcium-dependent inactivation. Abderemane-Ali, F., Findeisen, F., Rossen, N.D., and Minor, D.L. Jr., Neuron 101 1134-1149 (2019) PMID: 30733149 PMCID.
Structure of the saxiphilin:saxitoxin (STX)complex reveals a convergent molecular recognition strategy for paralytic toxins. Yen, T.-J., Lolicato, M., Thomas-Tran, R., Du Bois, J., and Minor, D.L. Jr., Science Advances 5 eaax2650 (2019) PMID: 31223657 PMCID: PMC6584486
Global versus local mechanisms of temperature sensing in ion channels. Arrigoni, C. and Minor, D.L. Jr., Pflügers Archiv: European Journal of Physiology 470 733-744 (2018) PMID: 29340775 PMCID: PMC5945320
A Calmodulin C-Lobe Ca2+-Dependent Switch Governs Kv7 Channel Function. Chang, A., Abderemane-Ali, F., Hura, G.L., Rossen, N.D., Gate, R.E., Minor, D.L., Jr., Neuron 97 836-852 (2018) View Video Abstract PMID:29429937 PMCID: PMC5823783
K2P2.1(TREK-1):activator complexes reveal a cryptic selectivity filter binding site. Lolicato, M., Arrigoni, C., Mori, T., Sekioka, Y., Bryant, C., Clark, K.A., Minor, D.L., Jr.,Nature 547 364-368 (2017) PMID: 28693035 PMCID: PMC5778891
Stapled voltage-gated calcium channel (CaV) α-Interaction Domain (AID) peptides act as selective protein-protein interaction inhibitors of CaV Function. Findeisen, F., Campiglio, M., Jo, H., Abderemane-Ali, F., Rumpf, C.H., Pope, L., Rossen, N.D., Flucher, B.E., DeGrado, W.F., and Minor D.L., Jr., ACS Chemical Neuroscience 8 1313-1326 (2017) PMID: 28278376 PMCID: PMC5481814
Unfolding of a temperature-sensitive domain controls voltage-gated channel activation. Arrigoni, C., Rohaim, A., Shaya, D., Findeisen, F., Stein, R.A. Nurva, S.R., Mishra, S., Mchaourab, H.S., and Minor, D.L., Jr., Cell 164 922-936 (2016)
Bacterial voltage-gated sodium channels (BacNaVs) from the soil, sea, and salt lakes enlighten molecular mechanisms of electrical signaling and pharmacology in the brain and heart. Payandeh, J. and Minor, D.L. Jr., Journal of Molecular Biology 427 3-30 (2015) PMID: 25158094 PMCID: PMC4277928