Home of the Electrosome

THE MINOR LAB

Home of the Electrosome We run on electricity.

Bioelectricity made by ion channel proteins drives our thoughts, feelings, and actions. These complex macromolecular devices give the spark to life by controlling the passage of ions across cell membranes in nerves, muscles, and the brain. Our laboratory focuses on using functional, chemical, and structural approaches to uncover the molecular mechanisms by which diverse types of ion channels work and to develop new reagents that can manipulate ion channel function. We are particularly interested in ion channels that respond to physical forces and that are important in pain and sensory physiology.

Latest Publications

Structural basis for activity and specificity of an anticoagulant anti-FXIa monoclonal antibody and a reversal agent. Ely, L., Lolicato, M. David, T., Lowe, K., Kim, Y.C., Samuel, D., Bessette, P., Garcia, J.L., Mikita, T., Minor, D.L. Jr., Coughlin, S.R., Structure 26187-198 (2018) 

A Calmodulin C-Lobe Ca2+-Dependent Switch Governs Kv7 Channel Function. Chang, A., Abderemane-Ali, F., Hura, G.L., Rossen, N.D., Gate, R.E., Minor, D.L., Jr., Neuron 97 836-852 (2018) View Video Abstract

Global versus local mechanisms of temperature sensing in ion channels. Arrigoni, C., Minor, D.L., Jr., Pflügers Archiv-European Journal of Physiology 1-12 (2018) ONLINE

Cryo-EM structures of the TMEM16A calcium-activated chloride channel. Dang S., Feng S., Tien J., Peters C.J., Bulkley D., Lolicato M., Zhao J., Zuberbühler K., Ye W., Qi L., Chen T., Craik C.S., Nung Jan Y., Minor D.L. Jr, Cheng Y., Yeh Jan L., Nature 552 426-429 (2017)

K2P2.1(TREK-1):activator complexes reveal a cryptic selectivity filter binding site. Lolicato, M., Arrigoni, C., Mori, T., Sekioka, Y., Bryant, C., Clark, K.A., Minor, D.L., Jr., Nature 547 364-368 (2017)

Stapled voltage-gated calcium channel (CaV) α-Interaction Domain (AID) peptides act as selective protein-protein interaction inhibitors of CaV Function. Findeisen, F., Campiglio, M., Jo, H., Abderemane-Ali, F., Rumpf, C.H., Pope, L., Rossen, N.D., Flucher, B.E., DeGrado, W.F., and Minor D.L., Jr., ACS Chemical Neuroscience 8 1313-1326 (2017)

Unfolding of a temperature-sensitive domain controls voltage-gated channel activation.  Arrigoni, C., Rohaim, A., Shaya, D., Findeisen, F., Stein, R.A. Nurva, S.R., Mishra, S., Mchaourab, H.S., and Minor, D.L., Jr., Cell 164 922-936 (2016) 

Journal of Molecular Biology
Journal of Molecular Biology
Journal of Molecular Biology

MINOR LAB RESEARCH

Our lab employs a range of biochemical, biophysical, structural, and chemical biology approaches to examine the molecular structures and functions of various classes of ion channels including members of the voltage-gated potassium, voltage-gated calcium, voltage-gated sodium, and K2P channel families. Our research relies heavily on X-ray crystallography, cryo-electron microscopy, isothermal titration calorimetry, circular dichroism, and other biophysical methods. Because ion channel structure is intimately tied to function, an equally crucial part of our efforts implements structure-based tests of ion channel function using electrophysiological recordings in live cells.

Because most channels suffer from poor pharmacological profiles that limit the ability to connect ion channel genes with their physiological functions, our lab also has a strong effort to develop novel ion channel modulators. The development of new selective inhibitors and activators of channel function should provide new tools for ion channel research and may lead to the development of novel, ion channel directed pharmaceuticals.

Transformation

MINOR LAB LIFE

The Minor lab is located in the Smith Cardiovascular Research Building at the UCSF Mission Bay Campus in San Francisco. If you are interested in joining us, please see here.

Affiliate UCSF Graduate Programs